Run BLAST against Local Database

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

💡 You can paste sequences with or without FASTA headers. Both formats are supported:

Raw sequence: MKFFLLFLVVLPIMGALGKK...
FASTA format: >sequence_name MKFFLLFLVVLPIMGALGKK...

Enter query sequence:

Upload FASTA file:

Choose File

Supported formats: .fasta, .fa, .txt

Query sequence type:

BLAST against:

Algorithm parameters

E-value threshold: Lower values = more stringent (min: 0.001)

Max target sequences: Maximum number of hits to return (1-10000)